1.1 This test method is designed as a simple and inexpensive initial screening procedure for new compounds with unknown pharmacological properties, or for the comparative bioassay of new members of a chemical series with class reference standards. The test method, which is applicable to most pharmacologically active compounds including pesticides, will properly rank order both acute lethality and potency with a minimum expenditure of test material. It is intended as the first step in a multi-tiered development program.

1.2 This standard does not purport to address the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.