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ASTM E2525-08
Standard Test Method for Evaluation of the Effect of Nanoparticulate Materials on the Formation of Mouse Granulocyte-Macrophage Colonies
5 стр.
Заменен
Электронный (pdf)Печатное издание
93.60 $ (включая НДС 20%)
Разработчик:
Зарубежные/ASTM
ICS:
07.100.10 Medical microbiology / Медицинская микробиология
Сборник (ASTM):
14.02 Particle and Spray Characterization; Forensic Sciences; Accreditation & Certification; Forensic Psychophysiology; Nanotechnology; Forensic Engineering / Характеристика гранул и аэрозолей; Судебные науки; Аккредитация и сертификация; Судебная психофизиология; Нанотехнологии; Судебная инженерия
Тематика:
Nanotechnology
Описание
Значение и использование

Stem cells of hematopoietic origin are pluripotential and may be particularly sensitive to the effects of stimulation by nanoparticulate materials.

The effect of particles on macrophage responses has an extensive history and can be assessed by Practice F 1903. The test method described here will assess the effect on stem cells which can be progenitor cells to the macrophage line.

Область применения

1.1 This test method provides a protocol for quantitative analysis of the effect of nanoparticulate materials in physiologic solution on granulocyte-macrophage colony-forming units.

1.2 This test method employs murine bone marrow hematopoietic stem cells which proliferate and differentiate to form discrete cell clusters or colonies which are counted.

1.3 This test method is part of the in vitro preclinical characterization cascade for nanoparticulate materials for systemic administration in medical applications.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Ключевые слова:
bone marrow; CFU-GM; clonogenic assay; granulocyte; macrophage; myelosuppression; nanoparticles; preclinical development; stem cells