5.1 This practice is intended to be used by all analysts using fused silica capillary chromatography. It contains the recommended steps for installation, preparation, proper installation, and continued column maintenance.
1.1 This practice covers the installation and maintenance of fused silica capillary columns in gas chromatographs that are already retrofitted for their use. This practice excludes information on:
1.2 For additional information on gas chromatography, please refer to Practice E260. For specific precautions, see 7.2.2.2(1), 7.2.2.2(2), 7.2.7, and 7.2.7.2.
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific safety information, see Section 6, 7.2.2.2(1), 7.2.2.2(2), 7.2.7, and 7.2.7.2.2
Carrier gas | 0.25-mm ID, | 0.32-mm ID, | 0.53-mm ID, |
helium | 35 | 80 | 125 |
| Oven temperature: 210°C isothermal |
| Injector and detector temperature: 250°C/300°C |
| ECD sensitivity: 512 × 10 −11 |
| Split vent: 100 cm 3 /min |
| 1. 2,4,5,6-tetrachloro- | 8. Heptachlor | 16. p,p-DDD |
| m-xylene (IS) | epoxide | 17. Endrin aldehyde |
| 2. α-BHC | 9. γ-chlordane | 18. Endosulfan sul- |
| 3. β-BHC | 10. Endosulfan I | fate |
| 4. γ-BHC | 11. α-chlordane | 19. p,p-DDT |
| 5. δ-BHC | 12. Dieldrin | 20. Endrin ketone |
| 6. Heptachlor | 13. p,p-DDE | 21. Methyoxychlor |
| 7. Aldrin | 14. Endrin | 22. Decachlorobi- |
|
| 15. Endosulfan II | phenyl (IS) |
Oven temperature: 150 to 275°C at 4°C/min, |
|
Injector temperature: 250°C | Detector temperature: 300°C |
Carrier gas: Helium |
|
Linear velocity: 40 cm/s (Flow rate: 10 cm 3 /min) |
|
ECD sensitivity: 8 × 10 −11 AFS |
|