This test method covers evaluating relative bioresistance of aqueous metalworking fluids by challenging them with a biological inoculum prepared from specific deteriorated metalworking fluid from the user's site.

Formerly under the jurisdiction of Committee E35 on Pesticides and Alternative Control Agents, this test method was withdrawn in April 2004 and replaced by Practice E 2275, for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance.

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1.1 This test method covers evaluating relative bioresistance of aqueous metalworking fluids by challenging them with a biological inoculum prepared from specific deteriorated metalworking fluid from the user's site.

1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.