This test method describes a procedure for the assessment and evaluation of the toxic characteristics of a test substance that is applied daily to the skin of experimental animals for 90 days.

Formerly under the jurisdiction of Committee E47 on Biological Effects and Environmental Fate, this test method was withdrawn in February 2010, because other guidelines currently exist.

This test method provides information on health hazards likely to arise from repeated exposure to a pesticide or other chemical by the dermal route over a short time period. It may provide information needed to establish safety criteria for human exposure.

Signs of toxicity other than lethality can be observed.

This method can provide information on target organs and possible cumulative effects. It may aid in the selection of doses for chronic studies.

1.1 This test method describes a procedure for the assessment and evaluation of the toxic characteristics of a test substance that is applied daily to the skin of experimental animals for 90 days.

1.2 This test method is not capable of determining effects that have a long latency period (for example, carcinogenicity) or are life shortening.

1.3 This test method is intended primarily to be used with rats, guinea pigs, or rabbits. Other species may be used with appropriate modifications.

1.4 This standard does not purport to address the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.