The procedure in this test method should be used to evaluate the activity of the test formulation in reducing the bacterial population of the hands immediately after a single use and to determine persistent activity (inhibition of growth) after 6 h. Optionally, measurements of persistent activity after a 3 h period and measurements of cumulative activity may be made after repetitive uses over a five day period.

1.1 This test method is designed to measure the reduction of microbial flora on the skin. It is intended for determining both immediate and persistent microbial reductions, after single or repetitive treatments, or both. It may also be used to measure cumulative antimicrobial activity after repetitive treatments.

1.2 A knowledge of microbiological techniques is required for these procedures.

1.3 In this method, metric units are used for all applications, except for distance, in which case inches are used and metric units follow in parentheses.

1.4 Performance of this procedure requires the knowledge of regulations pertaining to the protection of human subjects.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.