Formerly under the jurisdiction of Committee F04 on Medical and Surgical Materials and Devices, this guide was withdrawn in October 2013. This standard is being withdrawn without replacement because more detailed methods have since been developed and approved.

Micronucleus assays for genetic damage have been developed in many types of eucaryotic cells, both in vitro and in vivo. The occurrence of micronuclei is indicative of chromosomal damage or mitotic spindle dysfunction.

1.1 This guide covers minimal criteria which should be met by a micronucleus assay system prior to the development of an ASTM Standard or Guide for the conduct of that assay.

1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.