Область применения

1.1 This practice covers procedures for obtaining data concerning toxicity and other effects of a test material to a multi-trophic level freshwater community.

1.2 These procedures also might be useful for studying the fate of test materials and transformation products, although modifications and additional analytical procedures might be necessary.

1.3 Modification of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting multi-trophic level tests.

1.4 This practice is arranged as follows:

SectionReferenced Documents2Terminology3Summary of Practice4Significance and Use5Apparatus6Facilities6.1Container6.2Equipment6.3Hazards7Microcosm Components8Medium8.1Medium Preparation8.2Sediment8.3Microcosm Assembly8.4Test Material9General9.1Stock Solution9.2Nutrient Control9.3Test Concentration(s)9.4Test Organisms10Algae10.1Animals10.2Specificity of Organisms10.3Sources10.4Algal Culture Maintenance10.5Animal Culture Maintenance10.6SectionProcedure11Experimental Design11.1ProcedureInoculation11.2Culling11.3Addition of Test Material11.4Measurements11.5Reinoculations11.6Analytical Methodology12Data Processing13Calculations of Variables from Measurements14Statistical Analyses15Acceptability of Test16Interpretation of Results17Report18AnnexAnnex A1AppendicesRelationship of MediaAppendix X1Data SheetsAppendix X2Statistical GuidanceAppendix X3

1.5 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 7.