This guide is intended for use by individuals maintaining and handling hazardous biological material in liquid nitrogen freezers.

This guide does not cover all aspects of every situation that may be encountered in maintaining hazardous biological material in liquid nitrogen; each situation must therefore be assessed individually using these guidelines.

This guide is not intended for use with systems other than liquid nitrogen storage.

This guide does not cover practices for preservation by freezing which are covered in Practice E 1342.

1.1 This guide covers recommended procedures for maintaining and handling hazardous biological materials at liquid nitrogen temperatures.

1.2 This guide covers the safety precautions recommended when handling material stored in liquid nitrogen.

1.3 This guide does not cover the maintenance and handling of hazardous biological materials maintained at cryogenic temperatures in systems other than liquid nitrogen.

1.4 The values stated in SI units are to be regarded as the standard.

This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.