This test method is designed to demonstrate the effectiveness of an antibacterial wash product in reducing the resident microbial flora or a marker organism (representing transients) when used as recommended. Microbial activity can be compared with either a bland soap control or to a baseline organism count. Microbial samples can be collected either manually or by the mechanical Thran spray gun sampler.

Formerly under the jurisdiction of Committee E35 on Pesticides and Alternative Control Agents, this test method was withdrawn in January 2006 in accordance with section 10.5.3.1 of the Regulations Governing ASTM Technical Committees, which requires that standards shall be updated by the end of the eighth year since the last approval date.

1.1 This test method is designed to demonstrate the effectiveness of an antibacterial wash product in reducing the resident microbial flora or a marker organism (representing transients) when used as recommended. Microbial activity can be compared with either a bland soap control or to a baseline organism count. Microbial samples can be collected either manually or by the mechanical Thran spray gun sampler.

1.2 A knowledge of microbiological techniques is required for these procedures.

1.3 In this test method, metric units are used for all applications.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

1.5 It is the responsibility of the invesigator to determine if Good Laboratory Practice (GLP) and Good Clinical Practice (GLP) and Good Clinical Practice (GCP) is required.