The determination of endotoxin concentrations in metalworking fluids is a parameter that can be used in decision-making for prudent fluid management practices (fluid draining, cleaning, recharging or biocide dosages).

This standard provides a test method for analysts who perform quantitative endotoxin analyses of water-miscible metalworking fluids.

1.1 This test method covers quantitative methods for the sampling and determination of bacterial endotoxin concentrations in water miscible metalworking fluids (MWF).

1.2 Users of this test method need to be familiar with the handling of MWF.

1.3 This method gives an estimate of the endotoxin concentration in the sampled MWF.

1.4 This method replaces Test Method E2250.

1.5 This test method seeks to minimize inter-laboratory variation of endotoxin data but does not ensure uniformity of results.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.