This test method determines whether a test substance will elicit delayed-contact hypersensitivity in guinea pigs. The test method is applicable to individual chemical compounds, simple and complex mixtures, and also finished products.

Formerly under the jurisdiction of Committee E47 on Biological Effects and Environmental Fate, this test method was withdrawn in February 2010, because other guidelines currently exists.

This test method can be used to determine whether a test material will elicit skin sensitization or delayed contact
hypersensitivity in guinea pigs.^{3 ,4 ,5 ,6}

The results of this test method are a good indication of the potential for a given test material to produce delayed contact hypersensitivity in human beings under the appropriate conditions.

This test method can be used as a screening technique before conducting a similar test with human subjects.

1.1 This test method determines whether a test substance will elicit delayed-contact hypersensitivity in guinea pigs. The test method is applicable to individual chemical compounds, simple and complex mixtures, and also finished products.

1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.