This European Standard specifies methods for the determination of residues of pesticides and polychlorinated
biphenyls (PCBs) in fatty food.
Each method described in this European Standard is suitable for identifying and quantifying a definite range of
those non-polar organochlorine and/or organophosphorus pesticides which occur as residues in fats and oils as
well as in the fat portion of fat-containing foodstuffs, both of either animal or vegetable origin. The PCB
indicator congeners usually selected for the enforcement of maximum residue lirnits (MRLs) are determined
along with the organochlorine pesticides.
This European Standard contains the following clean-up methods that have been subjected to interlaboratory
studies and are adopted throughout Europe:
- Method A: Liquid-liquid partitioning with acetonitrile and clean-up on a Florisil@) column (AOAC) [ l ]
- Method B: Liquid-liquid partitioning with dimethylformamide and clean-up on a FlorisildDc olumn (Specht) [21
- Method C: Column chromatography on activated FlorisiP (AOAC) [3]
- Method D: Column chrornatography on partially deactivated Florisile (Stijve) 141
- Method E: Column chromatography on partially deactivated aluminium oxide (Greve & Grevenstuk) I51
- Method F: Gel permeation chromatography (GPC) (AOAC) [61
- Method G: Gel permeation chromatography (GPC) and colurnn chromatography on partially deactivated
silica gel (Specht) f 71
- Method H: High performance gel perrneation chromatography (HPGPC) (MAFF) 181
The applicability of the eight methods A to H for residue analysis of organochlorine pesticides, PCB indicator
congeners, and organophosphorus pesticides, respectively, is given in table A. 1. Where no + sign is shown,
there are no data avatlable in literature, but this does not necessarily exclude the applicability.
Diese ÖNORM beschreibt folgende Verfahren zur Bestimmung von Pestiziden und polychlorierten Biphenylen (PCB) in fettreichen Lebensmitteln: - Flüssig-Flüssig-Verteilung mit Acetonitril und Reinigung an einer Florisil-Säule - Flüssig-Flüssig-Verteilung mit Dimethylformamid und Reinigung an einer Florisil-Säule - Säulenchromatographie an aktiviertem Florisil - Säulenchromatographie an teilweise desaktiviertem Florisil- Säulenchromatographie an teilweise desaktiviertem Aluminiumoxid - Gelpermeations-Chromatographie - Gelpermeations-Chromatographie und Säulenchromatographie an teilweise desaktiviertem Kieselgel- Hochleistungs-Gelpermeations-Chromatographie